Electroporation and Its Effects on Endothelial Cells
Author Information
Author(s): Cemazar M, Parkins C S, Holder A L, Chaplin D J, Tozer G M, Sersa G
Primary Institution: Department of Tumor Biology, Institute of Oncology, Ljubljana, Slovenia
Hypothesis
The study aims to evaluate the cytotoxicity of electroporation with bleomycin or cisplatin on cultured human microvascular endothelial cells.
Conclusion
Electroporation significantly increases the cytotoxicity of bleomycin and cisplatin on endothelial cells.
Supporting Evidence
- Electroporation increased cytotoxicity of cisplatin by ∼10-fold.
- Electroporation increased cytotoxicity of bleomycin by ∼5000-fold.
- Electroporation of HMEC-1 cells with bleomycin was ∼250-fold better than continuous exposure.
Takeaway
This study shows that using electricity with certain cancer drugs can make them much more effective at killing cancer cells.
Methodology
The sensitivity of HMEC-1 cells to electroporation with bleomycin or cisplatin was compared to continuous drug exposure.
Limitations
Further studies are necessary to identify the determinants of endothelial response and its relationship to the anti-vascular action of electrochemotherapy in vivo.
Digital Object Identifier (DOI)
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