The Marine-Derived Oligosaccharide Sulfate (MdOS), a Novel Multiple Tyrosine Kinase Inhibitor, Combats Tumor Angiogenesis both In Vitro and In Vivo
2008

New Tyrosine Kinase Inhibitor

publication 10 minutes Evidence: moderate

Author Information

Author(s): Ma Jingui, Xin Xianliang, Meng Linghua, Tong Linjiang, Lin Liping, Geng Meiyu, Ding Jian

Primary Institution: Shanghai Institute of Materia Medica, Chinese Academy of Sciences

Hypothesis

The aim of the present study was to identify novel multi-targeting PTK inhibitors.

Conclusion

MdOS, a novel marine-derived oligosaccharide sulfate, exhibited anti-angiogenic activity in a PTK-dependent manner and is a promising agent for cancer therapy.

Supporting Evidence

  • MdOS inhibited the kinase activities of HER-2, EGFR, and VEGFR2 with IC50 values of 0.13, 0.28, and 1.8 µg/ml, respectively.
  • MdOS blocked tyrosine kinase phosphorylation and downstream signaling in various cancer cell lines.
  • In vivo studies showed that MdOS significantly inhibited neovascularization in a chicken chorioallantoic membrane model.

Takeaway

MdOS is a new type of medicine made from sea ingredients that helps stop cancer by blocking certain proteins that help tumors grow.

Methodology

The study used enzyme-linked immunosorbent assay (ELISA), sulforhodamine (SRB) assay, western blot analysis, and various in vitro and in vivo assays to evaluate the effects of MdOS.

Potential Biases

Potential bias may arise from the use of specific cell lines and animal models that may not fully represent human cancer biology.

Limitations

The study does not address the long-term effects of MdOS or its efficacy in human subjects.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003774

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