Immunohistochemical detection of mutant p53 protein in epithelial ovarian cancer using polyclonal antibody CMI: correlation with histopathology and clinical features
1994

Detection of Mutant p53 Protein in Ovarian Cancer

Sample size: 50 publication Evidence: moderate

Author Information

Author(s): J. Renninson, B.W. Baker, A.T. McGown, D. Murphy, J.D. Norton, B.W. Fox, D. Crowther

Primary Institution: Paterson Institute for Cancer Research, Christie Hospital NHS Trust

Hypothesis

Is there a correlation between mutant p53 protein presence and clinicopathological features in ovarian adenocarcinoma?

Conclusion

The study found a significant difference in p53 positivity between serous and mucinous ovarian tumors, but no correlation with treatment response or differentiation stage.

Supporting Evidence

  • 56% of the ovarian adenocarcinoma cases examined were p53 positive.
  • 18 out of 23 serous tumors were p53 positive compared to 3 out of 12 mucinous tumors.
  • There was no significant correlation between p53 status and differentiation stage or response to treatment.

Takeaway

The study looked at 50 ovarian cancer samples to see if a specific protein, p53, was present and found that it was more common in one type of tumor than another.

Methodology

The study used immunohistochemical staining and direct nucleotide sequencing to analyze p53 expression in tumor samples.

Limitations

The study had a small number of early-stage cases, limiting the ability to draw conclusions about p53 status in those stages.

Participant Demographics

Patients undergoing laparotomy for epithelial ovarian cancer in the north-west of England.

Statistical Information

P-Value

0.0237

Statistical Significance

p=0.0237

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