Impact of Staphylococcus aureus Biofilm on Human Skin Cells
Author Information
Author(s): Patrick R. Secor, James Garth, Philip Fleckman, John E. Olerud, Kate McInnerney, Philip S. Stewart
Primary Institution: Center for Biofilm Engineering, Montana State University
Hypothesis
How do biofilm and planktonic cultures of Staphylococcus aureus affect gene expression and cytokine production in human keratinocytes?
Conclusion
Staphylococcus aureus biofilms induce a distinct inflammatory response in human keratinocytes compared to planktonic cultures.
Supporting Evidence
- Biofilm-conditioned medium (BCM) upregulated 65 transcripts and downregulated 247 transcripts in keratinocytes.
- Cytokine production was higher in planktonic cultures after 24 hours compared to biofilm cultures.
- BCM induced apoptosis in keratinocytes, while PCM did not.
Takeaway
This study shows that bacteria in biofilms behave differently than those in liquid, affecting how our skin cells respond to them.
Methodology
Human keratinocytes were exposed to biofilm-conditioned medium (BCM) and planktonic-conditioned medium (PCM) for analysis of gene expression and cytokine production.
Limitations
The study primarily focuses on in vitro responses, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p < 0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website