Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413
2006

Study of Trichoderma harzianum CECT 2413 Genome

Sample size: 8710 publication 10 minutes Evidence: high

Author Information

Author(s): Vizcaíno Juan Antonio, González Francisco Javier, Suárez M Belén, Redondo José, Heinrich Julian, Delgado-Jarana Jesús, Hermosa Rosa, Gutiérrez Santiago, Monte Enrique, Llobell Antonio, Rey Manuel

Primary Institution: IBVF-CIC Isla de la Cartuja, CSIC/Universidad de Sevilla

Hypothesis

What genes are involved in the biological control activities of Trichoderma harzianum?

Conclusion

The EST collection and its annotation provide a significant resource for research on T. harzianum, a fungus with high biotechnological interest.

Supporting Evidence

  • 3,478 unique sequences were identified from 8,710 ESTs generated.
  • 51.1% of unique sequences were annotated using Gene Ontology.
  • 800 predicted proteins were identified with a putative signal peptide.

Takeaway

Scientists studied a fungus called Trichoderma harzianum to find out what genes help it fight off plant diseases. They found a lot of useful information about its genes.

Methodology

Eight different cDNA libraries were constructed from T. harzianum under various growth conditions, and 8,710 ESTs were generated and analyzed.

Limitations

The study only represents a portion of the T. harzianum genome and may not capture all unique sequences.

Digital Object Identifier (DOI)

10.1186/1471-2164-7-193

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