Gallbladder-Specific Regulatory Sequence of Sox17
Author Information
Author(s): Zeng Shihan, Yanagida Ayaka, Ota Noriaki, Uemura Mami, Hirate Yoshikazu, Hiramatsu Ryuji, Mizuno Naoaki, Kanai Yoshiakira, Kanai‐Azuma Masami
Primary Institution: The University of Tokyo
Hypothesis
What are the regulatory mechanisms underlying the time- and tissue-specific expression of Sox17?
Conclusion
The distal regulatory sequences of Sox17 are crucial for its expression in the embryonic gallbladder and for normal gallbladder epithelial development.
Supporting Evidence
- Sox17 Δdr/Δdr mice were alive and fertile, indicating that the deletion did not affect overall viability.
- SOX17 expression in Sox17 Δdr/Δdr embryos was reduced to less than 50% of wild-type levels in the gallbladder epithelium.
- The study identified two putative regulatory sequences adjacent to the Sox17 transcription start sites.
Takeaway
This study found that certain parts of the Sox17 gene help control how it works in the gallbladder during development, and without them, the gallbladder doesn't develop properly.
Methodology
The study involved generating deletion mice for the Sox17 distal regulatory sequences using CRISPR/Cas9 genome editing and analyzing their gallbladder development.
Limitations
The study primarily focused on the gallbladder and did not explore the full range of Sox17 functions in other tissues.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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