Analysis of Myc-Induced Histone Modifications on Target Chromatin
Author Information
Author(s): Francesca Martinato, Matteo Cesaroni, Bruno Amati, Ernesto Guccione
Primary Institution: Department of Experimental Oncology, European Institute of Oncology (IEO), IFOM-IEO Campus, Milan, Italy
Hypothesis
Whether Myc dynamically regulates other histone modifications at its binding sites remains to be addressed.
Conclusion
Myc promotes acetylation of multiple lysines on histones H3 and H4, contributing to transcriptional activation at target promoters.
Supporting Evidence
- Myc binding promoted acetylation of multiple lysines, primarily of H3K9, H3K14, H3K18, H4K5 and H4K12.
- Dimethylation of H3K79 was also selectively induced at target promoters.
- A majority of target promoters showed co-induction of multiple marks correlating with recruitment of HATs.
- Myc selectively induces H2A.Z incorporation in its target promoters.
Takeaway
Myc helps change the way certain genes are turned on by adding special tags to proteins called histones, making it easier for the cell to read those genes.
Methodology
Quantitative chromatin immunoprecipitation (qChIP) was used to profile histone modifications in a human B-cell line with a regulatable c-myc transgene.
Potential Biases
Potential bias due to the use of a single cell line and specific experimental conditions.
Limitations
The study does not address the effects of Myc on histone modifications in a broader context beyond the specific cell line used.
Participant Demographics
Human B-cell line P493-6.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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