Prolactin Receptor Expression in Canine Reproductive Tissues
Author Information
Author(s): Kowalewski Mariusz P, Michel Erika, Gram Aykut, Boos Alois, Guscetti Franco, Hoffmann Bernd, Aslan Selim, Reichler Iris
Primary Institution: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Switzerland
Hypothesis
The expression of prolactin receptors (PRLr) in canine reproductive tissues varies during dioestrus and pregnancy.
Conclusion
The study suggests that prolactin plays a significant role in regulating both luteal and placental functions in dogs.
Supporting Evidence
- PRLr expression was significantly upregulated at day 15 post ovulation in non-pregnant dogs.
- Pregnant dogs showed elevated PRLr expression until mid-gestation.
- Placental PRLr expression was strongly time-related, with significant upregulation towards mid-gestation.
- Antigestagen treatment significantly downregulated PRLr in both luteal and placental tissues.
Takeaway
This study looked at how a hormone called prolactin works in dogs during pregnancy and after ovulation, showing that it helps keep the pregnancy going.
Methodology
The study used Real Time PCR, immunohistochemistry, and in situ hybridization to assess PRLr expression in various reproductive tissues.
Potential Biases
Potential bias due to the limited number of breeds and age range of dogs used in the study.
Limitations
The study did not explore the long-term effects of altered PRLr expression on reproductive outcomes.
Participant Demographics
Clinically healthy and sexually mature bitches of various breeds, aged 2-8 years.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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