Early detection of oral cancer: PAP and AgNOR staining in brush biopsies
2010

Early Detection of Oral Cancer Using PAP and AgNOR Staining

Sample size: 44 publication 10 minutes Evidence: high

Author Information

Author(s): Dinesh V Rajput, Jagdish V Tupkari

Primary Institution: Government Dental College and Hospital, Mumbai

Hypothesis

The study aims to determine the diagnostic accuracy of routine Papanicolaou stain (PAP) and AgNOR staining in brush biopsies for early detection of oral cancer.

Conclusion

Brush biopsy combined with AgNOR staining is a reliable and non-invasive method for early detection of oral cancerous lesions.

Supporting Evidence

  • The sensitivity of PAP analysis for detecting oral cancer was 91.176% and specificity was 100%.
  • AgNOR analysis showed 100% sensitivity and specificity for detecting oral cancer.
  • Positive predictive value for both PAP and AgNOR was 100%.

Takeaway

Doctors can use a simple brush to collect cells from the mouth and then stain them to check for cancer, which is easier and safer than traditional methods.

Methodology

Brush biopsies were taken from 34 patients with suspicious oral lesions and 10 control subjects, followed by PAP and AgNOR staining for analysis.

Limitations

Further investigations with a larger sample size are needed to confirm the findings.

Participant Demographics

34 patients with suspected oral cancer and 10 normal-aged and sex-matched controls.

Statistical Information

P-Value

<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.4103/0973-029X.72501

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