SH2-Inositol Phosphatase 1 Negatively Influences Early Megakaryocyte Progenitors SHIP-Deficient Thrombopoiesis
2008

How SHIP Affects Blood Cell Development

Sample size: 4 publication 10 minutes Evidence: moderate

Author Information

Author(s): Perez Lia E., Desponts Caroline, Parquet Nancy, Kerr William G.

Primary Institution: H. Lee Moffitt Comprehensive Cancer Center and Research Institute

Hypothesis

SHIP might control megakaryocytopoiesis through effects on proliferation of megakaryocyte progenitors and megakaryocytes.

Conclusion

SHIP may play an important role in regulation of essential signaling pathways that control early megakaryocytopoiesis in vivo.

Supporting Evidence

  • SHIP deficient mice have increased numbers of megakaryocyte progenitors in hematopoietic organs.
  • Despite increased progenitor numbers, mature megakaryocyte numbers are not significantly changed.
  • SHIP deficient megakaryocyte progenitors exhibit increased phosphorylation of key signaling proteins.

Takeaway

This study found that a protein called SHIP helps control the development of certain blood cells, and when it's missing, there are more early blood cell precursors but not more mature blood cells.

Methodology

The study used flow cytometry and functional assays to analyze megakaryocyte progenitors in SHIP deficient mice.

Limitations

The study primarily focused on two strains of SHIP deficient mice, which may limit the generalizability of the findings.

Participant Demographics

The study involved six to eight week-old adult mice.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003565

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