Influenza A Virus NS1 Protein and Its Role in Cell Death
Author Information
Author(s): Zhang Chuanfu, Yang Yutao, Zhou Xiaowei, Yang Zhixin, Liu Xuelin, Cao Zhiliang, Song Hongbin, He Yuxian, Huang Peitang
Primary Institution: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, P.R. China
Hypothesis
The NS1 protein of influenza A virus interacts with heat shock protein Hsp90, affecting apoptosis in human alveolar basal epithelial cells.
Conclusion
The NS1 protein of Influenza A Virus interacts with heat shock protein Hsp90 and mediates apoptosis through the caspase cascade.
Supporting Evidence
- The NS1 protein was shown to induce apoptosis in human alveolar basal epithelial cells.
- Hsp90 was identified as a binding partner of the NS1 protein.
- The interaction between NS1 and Hsp90 affects the binding of Apaf-1 and Cyt c.
Takeaway
The NS1 protein from the flu virus can make cells die by messing with a helper protein called Hsp90.
Methodology
The study used a two-hybrid system and co-immunoprecipitation assays to identify interactions between NS1 and Hsp90 in A549 cells.
Digital Object Identifier (DOI)
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