How Multiple ETS Proteins Control PF4 Gene Expression
Author Information
Author(s): Okada Yoshiaki, Nobori Haruaki, Shimizu Mikiko, Watanabe Miho, Yonekura Masaaki, Nakai Tomoko, Kamikawa Yuko, Wakimura Atsuko, Funahashi Nobuaki, Naruse Hiroki, Watanabe Ayako, Yamasaki Daisuke, Fukada So-ichiro, Yasui Kazuta, Matsumoto Kayoko, Sato Takahiro, Kitajima Kenji, Nakano Toru, Aird William C., Doi Takefumi
Primary Institution: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
Hypothesis
The study investigates how multiple ETS family proteins regulate PF4 gene expression by binding to the same ETS binding site.
Conclusion
FLI-1, ELF-1, and GABP regulate PF4 gene expression through the −51 ETS site in megakaryocytes.
Supporting Evidence
- FLI-1, ELF-1, and GABP were shown to bind to the −51 ETS site.
- Mutation of the −51 ETS site significantly reduced promoter activation.
- siRNA analysis demonstrated that FLI-1, ELF-1, and GABP regulate PF4 gene expression.
Takeaway
This study found that certain proteins help control a gene important for blood cell development by sticking to a specific spot on the gene's instruction manual.
Methodology
The study used electrophoretic mobility shift assays, chromatin immunoprecipitation assays, and siRNA analysis to investigate protein binding and gene expression.
Limitations
The study primarily focused on in vitro assays, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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