MicroRNAs can regulate human APP levels
2008
MicroRNAs Regulate APP Levels
publication
Evidence: high
Author Information
Author(s): Neha Patel, David Hoang, Nathan Miller, Sara Ansaloni, Qihong Huang, Jack T Rogers, Jeremy C Lee, Aleister J Saunders
Primary Institution: Drexel University
Hypothesis
Can microRNAs regulate APP protein levels?
Conclusion
The study demonstrates that microRNAs hsa-mir-106a and hsa-mir-520c can negatively regulate human APP protein levels.
Supporting Evidence
- MicroRNAs hsa-mir-106a and hsa-mir-520c bind to the APP 3'UTR and reduce APP protein levels.
- Over-expression of these microRNAs led to a significant decrease in APP levels in human cell lines.
- Results were consistent across multiple experiments and cell types.
Takeaway
This study found that tiny molecules called microRNAs can help control the amount of a protein linked to Alzheimer's disease in human cells.
Methodology
The researchers used human cell lines to test the effects of specific microRNAs on APP levels through luciferase assays and Western blot analysis.
Statistical Information
P-Value
p < 0.01
Statistical Significance
p < 0.0001
Digital Object Identifier (DOI)
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