Update on Barley MicroRNAs and Their Targets
Author Information
Author(s): Colaiacovo Moreno, Subacchi Annalisa, Bagnaresi Paolo, Lamontanara Antonella, Cattivelli Luigi, Faccioli Primetta
Primary Institution: CRA-Genomics Research Centre
Hypothesis
To extend and update information on miRNAs and their targets in barley and to identify candidate polymorphisms at miRNA target sites.
Conclusion
This study has provided an update of the information on barley miRNAs and their targets, representing a foundation for future studies.
Supporting Evidence
- 156 microRNA mature sequences belonging to 50 miRNA families were found to significantly match at least one EST sequence in barley.
- Many previously known and several putatively new miRNA/target pairs have been identified.
- Candidate microRNA coding genes have been reported and genetic variation both in functional regions of putative miRNAs and at miRNA target sites has been found.
Takeaway
The study found many microRNAs in barley that help the plant grow and respond to stress, and it identified some genetic variations that could be useful for breeding.
Methodology
The study used computational methods to search for barley miRNA homologues and targets in publicly available ESTs, and statistical analysis to identify over and under-represented plant species.
Statistical Information
P-Value
0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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