In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites
2008
HPV-18E6 Fusion Protein and p53 Phosphorylation
publication
Evidence: moderate
Author Information
Author(s): Sun Lina, Zhang Ge, Li Zongfang, Song Tusheng, Huang Chen, Si Lusheng
Primary Institution: Xi'an Jiaotong University
Hypothesis
We investigated whether p53 could be phosphorylated by HPV-E6.
Conclusion
The study found that the HPV-18E6 fusion protein could transiently induce p53 phosphorylation at multiple sites in specific cell lines.
Supporting Evidence
- GFP-18E6 was predominantly located in nuclei of wt p53 cell lines.
- It could induce transient phosphorylation of p53 at multiple sites.
- Phosphorylated p53 was localized in nuclei together with GFP-18E6.
Takeaway
The HPV-18E6 protein can make a part of our body's defense system, called p53, work differently by adding special tags to it.
Methodology
The study used a GFP expression system to express HPV-18E6 in 293T and MCF-7 cells, followed by immunofluorescence and immunoblotting to analyze p53 phosphorylation.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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