How miR-93 and miR-155 Control AID in Breast Cancer Cells
Author Information
Author(s): Glen M Borchert, Nathaniel W Holton, Erik D Larson
Primary Institution: School of Biological Sciences, Illinois State University, Normal, IL, USA
Hypothesis
Can miR-93 and miR-155 regulate the expression of Activation Induced Cytidine Deaminase (AID) in breast cancer cells?
Conclusion
miR-93 and miR-155 suppress AID translation in MCF-7 cells, which may help prevent genomic instability and cancer progression.
Supporting Evidence
- miR-93 and miR-155 were found to have complementary target sites in the AID mRNA.
- Overexpression of miR-93 and miR-155 reduced AID protein levels in MCF-7 cells.
- Depletion of miR-93 or miR-155 increased AID protein levels without affecting AID mRNA levels.
Takeaway
This study found that two tiny molecules, miR-93 and miR-155, help keep a protein called AID from causing problems in breast cancer cells.
Methodology
The study used luciferase reporter assays and western blotting to evaluate the effects of miR-93 and miR-155 on AID expression in MCF-7 breast carcinoma cells.
Digital Object Identifier (DOI)
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