Hoechst 33342 and Tumour Perfusion
Author Information
Author(s): M.J. Trotter, P.L. Olive, D.J. Chaplin
Primary Institution: BC Cancer Research Centre
Hypothesis
What is the effect of the vascular marker Hoechst 33342 on tumour perfusion and cardiovascular function in mice?
Conclusion
Hoechst 33342 causes a transient decrease in tumour blood flow and arterial blood pressure in mice, but these effects do not preclude its use as a vascular marker.
Supporting Evidence
- H33342 caused a dose-dependent reduction in tumour red blood cell flow.
- The maximum decline in blood pressure was 20 ± 6 mmHg.
- Blood flow in foot tumours remained significantly depressed for only 2-3 minutes.
- H33342 had no significant effect on blood flow in skin of a non-tumour-bearing foot.
- Injection of H33342 20 minutes before irradiation had no influence on tumour radiation response.
Takeaway
The dye Hoechst 33342 can help scientists see blood flow in tumors, but it can also temporarily lower blood flow and pressure in mice.
Methodology
Mice with SCCVII tumours were injected with Hoechst 33342, and blood flow was measured using laser Doppler flowmetry.
Limitations
The study's findings may not apply to all tumor types or implantation sites, and high doses of H33342 should be avoided.
Participant Demographics
6-8-week-old male C3H/He mice
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
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