T Cell Repertoire Analysis in Diabetic Mice
Author Information
Author(s): Vong Allen M, Daneshjou Nazila, Norori Patricia Y, Sheng Huiming, Braciak Todd A, Sercarz Eli E, Gabaglia Claudia Raja
Primary Institution: Laboratory of Vaccine Research, Torrey Pines Institute for Molecular Studies
Hypothesis
B cell reconstitution in NOD.Igμnull mice induces a pathogenic T cell repertoire that leads to diabetes.
Conclusion
B cell reconstitution in NOD.Igμnull mice causes a polyclonal T cell accumulation in the pancreas, leading to diabetes.
Supporting Evidence
- B cell reconstitution leads to a significant increase in T cell receptor diversity in the pancreas.
- Diabetes onset in reconstituted mice occurs between 18 to 22 weeks post-reconstitution.
- The majority of infiltrating lymphocytes in the pancreas are CD19+ B cells.
Takeaway
When certain mice that usually don't get diabetes are given B cells, they start to develop diabetes because of the T cells that grow in their pancreas.
Methodology
Spectratyping analysis was performed on T cell receptor V beta expansions in NOD.Igμnull mice before and after B cell reconstitution.
Limitations
The study primarily focuses on a specific mouse model, which may not fully represent human diabetes.
Participant Demographics
NOD.Igμnull mice, specifically female, aged 4 weeks at the start of the study.
Digital Object Identifier (DOI)
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