Measuring Carbonic Anhydrase IX as a Hypoxia Biomarker
Author Information
Author(s): Tobias C Wind, Michael P Messenger, Douglas Thompson, Peter J Selby, Rosamonde E Banks
Primary Institution: Cancer Research UK Centre, Leeds Institute of Molecular Medicine, St James's University Hospital
Hypothesis
The study investigates the differences in concentrations of carbonic anhydrase IX in serum and plasma due to the influence of metal ions.
Conclusion
The study highlights the need for stringent validation of commercially available assays, as differences in CA IX concentrations between serum and plasma can lead to invalid conclusions in research.
Supporting Evidence
- Marked differences in CA IX concentrations were found between EDTA plasma and serum.
- One ELISA was successfully validated while the other showed significant issues with parallelism.
- Metal ions were found to influence the measurement of CA IX in serum and plasma.
Takeaway
This study looked at how measuring a protein called carbonic anhydrase IX in blood can change depending on whether the blood is taken as serum or plasma, which is important for cancer research.
Methodology
Matched serum and EDTA plasma samples from patients with renal cancer and healthy controls were analyzed using two commercially available ELISAs for CA IX.
Potential Biases
There may be risks of bias due to the differences in assay performance and sample handling.
Limitations
The study was limited by the variability in sample collection and the potential influence of metal ions on assay results.
Participant Demographics
17 patients with renal cancer (10 men, 7 women; age range 42–75 years) and 10 healthy controls (4 men, 6 women; age range 21–66 years).
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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