Production of Streptokinase in Streptomyces lividans
Author Information
Author(s): Elsa Pimienta, Julio C Ayala, Caridad Rodríguez, Astrid Ramos, Lieve Van Mellaert, Carlos Vallín, Jozef Anné
Primary Institution: Laboratorio de Genética, Centro de Química Farmacéutica, Ciudad de la Habana, Cuba; Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, Belgium
Hypothesis
Can Streptomyces lividans be used as a host for the recombinant production of streptokinase?
Conclusion
Streptomyces lividans can effectively produce streptokinase, achieving higher yields with the Sec pathway compared to the Tat pathway.
Supporting Evidence
- The yield of streptokinase was significantly higher when using the Sec pathway compared to the Tat pathway.
- The specific activity of the purified streptokinase was determined to be 2661 IU/mg protein.
- The study demonstrated that the recombinant streptokinase was biologically active.
Takeaway
Scientists found a way to make a medicine called streptokinase using a type of bacteria, which can help people with blood clots. They learned that one method works much better than another.
Methodology
The study involved cloning the skc-2 gene from Streptococcus equisimilis into Streptomyces lividans and testing two different signal sequences for protein secretion.
Limitations
The study did not compare the activity of streptokinase produced in Streptomyces lividans with that from other hosts under identical conditions.
Digital Object Identifier (DOI)
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