Absence of rearrangements in the BRCA2 gene in human cancers
2001
No BRCA2 Gene Rearrangements Found in Human Cancers
Sample size: 213
publication
Evidence: moderate
Author Information
Author(s): Chin S-F, Wang Q, Puisieux A, Caldas C
Primary Institution: University of Cambridge and Cambridge Institute for Medical Research
Hypothesis
Are large genomic rearrangements involved in BRCA2 mutations in human cancers?
Conclusion
The study found no genomic rearrangements in the BRCA2 gene in various human cancers.
Supporting Evidence
- Mutations of BRCA2 in sporadic breast and ovarian carcinomas are exceedingly rare.
- The study involved 130 primary breast cancers and 83 cancer cell lines.
- No genomic rearrangements were found in the BRCA2 gene.
Takeaway
Scientists looked at a lot of cancer samples and didn't find changes in a gene called BRCA2, which means another gene might be causing the cancer instead.
Methodology
Southern blots were performed on genomic DNA from 130 primary breast cancers and 83 cancer cell lines.
Digital Object Identifier (DOI)
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