Adhesive properties of Enterobacter sakazakii to human cells
Author Information
Author(s): Mange Jean-Philippe, Stephan Roger, Borel Nicole, Wild Peter, Kim Kwang Sik, Pospischil Andreas, Lehner Angelika
Primary Institution: Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich
Hypothesis
This study investigates the adherence ability of Enterobacter sakazakii strains to human epithelial and brain microvascular endothelial cells.
Conclusion
The study found that different E. sakazakii strains exhibit varying adherence patterns to human cells, with no clear relationship to the production of specific fimbriae.
Supporting Evidence
- 58% of E. sakazakii strains showed positive adhesion to HEp-2 cells.
- Adhesion was maximal during the late exponential phase of bacterial growth.
- Different adhesion patterns were observed, including diffuse and clustered adhesion.
Takeaway
The researchers looked at how well a type of bacteria called E. sakazakii sticks to human cells, which is important for understanding how it can make people sick.
Methodology
The study examined the adherence of 50 E. sakazakii strains to HEp-2, Caco-2, and HBMEC cell lines, assessing the effects of bacterial culture conditions on adherence.
Limitations
The study did not explore all potential factors influencing adherence and focused on a limited number of strains.
Participant Demographics
The strains included reference strains, human isolates, food isolates, and environmental isolates.
Digital Object Identifier (DOI)
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