Identifying Allergic Epitopes in a Fungal Allergen
Author Information
Author(s): Nair Smitha, Kukreja Neetu, Singh Bhanu Pratap, Arora Naveen
Primary Institution: Institute of Genomics and Integrative Biology (CSIR), Delhi, India
Hypothesis
The study aimed to identify the B cell epitopes of alcohol dehydrogenase allergen from Curvularia lunata using in-silico methods and immunoassay.
Conclusion
Four B cell epitopes of C. lunata ADH were identified, with Peptide P2 being a potential candidate for diagnosing allergic diseases.
Supporting Evidence
- The study identified six B cell and four T cell epitopes.
- Peptide P2 showed maximum IgE binding with patients' sera.
- The computational methods were validated using known allergens.
Takeaway
The researchers found parts of a fungus that can make people allergic, which could help in diagnosing allergies.
Methodology
B cell epitopes were predicted using sequence and structure-based methods, and immunoreactivity was assessed by ELISA with patients' sera.
Participant Demographics
Patients of asthma, rhinitis, or both were screened based on history and clinical investigations.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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