Involvement of tumor necrosis factor-α in the upregulation of CXCR4 expression in gastric cancer induced by Helicobacter pylori
2010
How H. pylori Causes Gastric Cancer by Increasing CXCR4 Expression
Sample size: 34
publication
Evidence: high
Author Information
Author(s): Zhao Chenghai, Lu Xiaomei, Bu Xianmin, Zhang Ning, Wang Wei
Primary Institution: China Medical University
Hypothesis
Is TNF-α involved in the induction of CXCR4 expression by H. pylori in gastric cancer?
Conclusion
H. pylori increases CXCR4 expression in gastric cancer through the action of TNF-α.
Supporting Evidence
- H. pylori-positive gastric cancers showed significantly higher levels of CXCR4 and TNF-α mRNA compared to H. pylori-negative ones.
- Spearman's rank correlation test indicated a positive correlation between CXCR4 and TNF-α levels in gastric cancer specimens.
- Treatment with infliximab, a TNF-α neutralizing antibody, significantly inhibited CXCR4 expression induced by H. pylori.
Takeaway
H. pylori, a bacteria that can cause stomach problems, makes a protein called CXCR4 more active in stomach cancer, and this happens because of another protein called TNF-α.
Methodology
Real-time PCR and Western blotting were used to measure mRNA and protein levels of CXCR4 and TNF-α in gastric cancer cells and tissues.
Participant Demographics
34 primary gastric cancer specimens from patients undergoing surgery.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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