How TLR2 and TLR4 Make Dendritic Cells Tolerant
Author Information
Author(s): Valerie Albrecht, Thomas PJ Hofer, Brian Foxwell, Marion Frankenberger, Loems Ziegler-Heitbrock
Primary Institution: Helmholtz Center München, German Research Center for Environmental Health
Hypothesis
Can TLR2 and TLR4 induce tolerance in human dendritic cells?
Conclusion
TLR-ligands can make dendritic cells tolerant to TNF gene expression by blocking signal transduction through IRAK-1.
Supporting Evidence
- DCs stimulated with TLR4 ligand LPS showed rapid TNF expression.
- Pre-culturing DCs with LPS reduced their response to subsequent stimulation.
- IRAK-1 levels were significantly lower in LPS-tolerant DCs.
Takeaway
When dendritic cells are exposed to certain signals too many times, they stop responding as strongly. This study shows that two specific signals can make them less active.
Methodology
Dendritic cells were generated from PBMC and stimulated with TLR ligands to assess TNF expression and IRAK-1 levels.
Participant Demographics
Healthy volunteers' blood was used to generate dendritic cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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