Chronic Sleep Deprivation Causes Anxiety, Depression and Impaired Gut Barrier in Female Mice
Author Information
Author(s): Li Lingyue, Meng Zilin, Huang Yuebing, Xu Luyao, Chen Qianling, Qiao Dongfang, Yue Xia
Primary Institution: Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China
Hypothesis
Chronic sleep deprivation (CSD) affects gut microbiota and metabolites, leading to anxiety and depression-like behaviors in female mice.
Conclusion
CSD induced anxiety/depression-like behavior, caused gut microbiota dysbiosis, altered fecal metabolism, and damaged the colon barrier in female mice.
Supporting Evidence
- CSD led to increased harmful bacteria and decreased beneficial bacteria in the gut.
- Histological analysis showed epithelial cell hyperplasia and reduced goblet cells in the colon.
- Behavioral tests indicated significant anxiety and depression-like behaviors in sleep-deprived mice.
Takeaway
When female mice don't get enough sleep, they can feel anxious and sad, and their gut health gets worse.
Methodology
The study used a 48-day sleep deprivation model on female C57BL/6J mice, assessing behavior through various tests and analyzing fecal samples for microbiome and metabolome changes.
Potential Biases
The study primarily used female mice, which may limit generalizability to male mice or other populations.
Limitations
The study focused only on fecal metabolomics and did not consider brain regions associated with anxiety and depression.
Participant Demographics
Six-week-old specific-pathogen-free female C57BL/6J mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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