Study of Human Tear Proteins
Author Information
Author(s): Green-Church Kari B., Nichols Kelly K., Kleinholz Nan M., Zhang Liwen, Nichols Jason J.
Primary Institution: The Ohio State University
Hypothesis
The study aims to examine the tear film proteome and the effects of different tear collection methods on the proteome.
Conclusion
The choice of tear collection method significantly impacts the proteins present in the tear film.
Supporting Evidence
- 97 unique proteins were observed across sampling and proteomic methods.
- 54 unique proteins were identified from Schirmer strips compared to 13 from capillary tubes.
- Secreted proteins were predominantly observed from tears collected by capillary.
Takeaway
This study looked at the proteins in human tears and found that how you collect the tears can change what proteins you find.
Methodology
Tear samples were collected from eight subjects using two different methods and analyzed using various proteomic techniques including SDS-PAGE and mass spectrometry.
Limitations
The study may not capture all proteins due to the high abundance of certain proteins masking others.
Participant Demographics
Six of the eight subjects were female and seven were Caucasian.
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