BKV Agnoprotein Interacts with α-Soluble N-Ethylmaleimide-Sensitive Fusion Attachment Protein, and Negatively Influences Transport of VSVG-EGFP
2011
BK Virus Agnoprotein and Its Interaction with α-SNAP
publication
Evidence: moderate
Author Information
Author(s): Johannessen Mona, Walquist Mari, Gerits Nancy, Dragset Marte, Spang Anne, Moens Ugo
Primary Institution: University of Tromsø, Tromsø, Norway
Hypothesis
What is the role of BK virus agnoprotein in cellular interactions and secretion processes?
Conclusion
BK virus agnoprotein interacts with α-SNAP and negatively influences the secretion of proteins in cells.
Supporting Evidence
- BKV agnoprotein and α-SNAP were found to partially co-localize in cells.
- A complex consisting of agnoprotein and α-SNAP could be co-immunoprecipitated from cells.
- The N-terminal part of the agnoprotein was sufficient for the interaction with α-SNAP.
- BKV agnoprotein negatively interferes with secretion of VSVG-EGFP reporter.
Takeaway
The BK virus has a protein that can stick to another protein in our cells, which stops things from being sent out of the cell.
Methodology
Yeast two-hybrid assays, co-immunoprecipitation, and confocal microscopy were used to study the interactions and localization of proteins.
Limitations
The interaction might not be direct and could involve other proteins.
Digital Object Identifier (DOI)
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