Probiotics and Inflammation in Crohn's Disease
Author Information
Author(s): Mencarelli Andrea, Distrutti Eleonora, Renga Barbara, D'Amore Claudio, Cipriani Sabrina, Palladino Giuseppe, Donini Annibale, Ricci Patrizia, Fiorucci Stefano
Primary Institution: University of Perugia
Hypothesis
Probiotics can modulate the expression of nuclear receptors in intestinal and adipose tissues in a rodent model of colitis and in Crohn's patients.
Conclusion
Probiotics can correct inflammation-driven metabolic dysfunction in mesenteric adipose tissue associated with Crohn's disease.
Supporting Evidence
- Probiotic administration reduced colonic expression of inflammatory cytokines.
- VSL#3 treatment prevented changes in nuclear receptor expression caused by TNBS.
- Mesenteric fat from TNBS-treated mice showed increased inflammatory mediators.
- Probiotics modulated leptin release from adipose tissue explants.
Takeaway
This study shows that probiotics can help reduce inflammation in the intestines and fat tissue of mice with a disease similar to Crohn's disease.
Methodology
Colitis was induced in mice using TNBS, and they were treated with probiotics daily for 10 days; human adipose tissue explants were cultured with probiotic medium.
Potential Biases
Potential bias in the selection of human samples and the interpretation of results from animal models.
Limitations
The study was conducted on a rodent model and human samples, which may not fully represent the complexity of Crohn's disease in all patients.
Participant Demographics
Five Crohn's disease patients (2 women, mean age 36) and five patients with colon cancer (2 women, mean age 47) were included.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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