Phosphorylation of Twist1 Family Transcription Factors in Heart Remodeling
Author Information
Author(s): Lu Shuangshuang, Nie Junwei, Luan Qing, Feng Qiuting, Xiao Qi, Chang Zai, Shan Congjia, Hess Daniel, Hemmings Brian A., Yang Zhongzhou
Primary Institution: The Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China
Hypothesis
How does phosphorylation of Twist1 family transcription factors affect post-natal heart function and remodeling?
Conclusion
Phosphorylation of the Twist1 family transcription factors is involved in pathological cardiac remodeling and can be regulated by Akt signaling.
Supporting Evidence
- Transgenic mice with over-expressed Hand1 and Twist1 mutants showed pathological cardiac remodeling.
- Gene expression analysis revealed up-regulation of growth-promoting genes and down-regulation of metabolic genes.
- Phosphorylation of Hand1 regulated its transcriptional activation and DNA binding ability.
Takeaway
This study shows that certain proteins in the heart can change how the heart grows and functions, especially when they are modified by a process called phosphorylation.
Methodology
Transgenic mice were generated to study the effects of over-expressing Hand1 and Twist1 mutants in cardiomyocytes, followed by gene expression analysis and biochemical assays.
Limitations
The study primarily uses mouse models, which may not fully replicate human heart conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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