The Pseudomonas putida Lon protease is involved in N-acyl homoserine lactone quorum sensing regulation
2007
Lon Protease in Pseudomonas putida and Quorum Sensing
Sample size: 25000
publication
Evidence: high
Author Information
Author(s): Bertani Iris, Rampioni Giordano, Leoni Livia, Venturi Vittorio
Primary Institution: International Centre for Genetic Engineering and Biotechnology
Hypothesis
Is the Lon protease involved in the regulation of AHL quorum sensing in Pseudomonas putida?
Conclusion
The Lon protease is a negative regulator of AHL production in P. putida WCS358.
Supporting Evidence
- Three Tn5 mutants showed overproduction of AHLs in P. putida strain WCS358.
- The lon protease mutant produced three times more C12-3-oxo-AHL.
- Restoring the lon protease in trans reduced AHL production back to wild-type levels.
Takeaway
Scientists found that a protein called Lon helps control how bacteria communicate with each other by regulating a special signal they produce.
Methodology
Screening of a Tn5 genomic mutant bank of P. putida to identify negative regulators of AHL quorum sensing.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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