From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in Monascus Species
2024
Review of Gene Function Study Methods in Monascus Species
publication
Evidence: moderate
Author Information
Author(s): Gong Yunxia, Li Shengfa, Zhao Deqing, Yuan Xi, Zhou Yin, Chen Fusheng, Shao Yanchun
Primary Institution: Wuhan Business University
Conclusion
This review summarizes various genetic manipulation tools for studying Monascus species, highlighting the importance of precise gene editing techniques.
Supporting Evidence
- Monascus species are important for producing bioactive compounds.
- Genetic manipulation tools can help optimize fermentation processes.
- CRISPR/Cas9 has revolutionized gene editing in fungi.
Takeaway
Scientists are finding better ways to change the genes in a type of fungus called Monascus, which can help make useful products like food colors and medicines.
Methodology
The review discusses various genetic transformation techniques including Agrobacterium tumefaciens-mediated transformation and CRISPR/Cas9 gene editing.
Limitations
The review does not provide specific experimental data or results from studies.
Digital Object Identifier (DOI)
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