Inhibitory Effect of LY 255283 on Leukotriene and Thromboxane Synthesis
Author Information
Author(s): M. UOur, M. MellicA
Primary Institution: Department of Pharmacology, Medical Faculty of Ankara University
Hypothesis
LY 255283 may inhibit the production of leukotriene B and thromboxane B2 by antagonizing the effect of ionophore-induced leukotriene B on specific enzymes in human immune cells.
Conclusion
LY 255283 significantly inhibited the synthesis of leukotriene B4 and thromboxane B2 in human peripheral blood polymorphonuclear leukocytes and monocytes.
Supporting Evidence
- LY 255283 inhibited the production of leukotriene B4 in human peripheral blood polymorphonuclear leukocytes.
- LY 255283 also inhibited the production of thromboxane B2 in monocytes activated by calcium ionophore.
- The study showed that the effect of LY 255283 was more prominent on the synthesis of leukotriene B4, especially in monocytes.
Takeaway
This study found that a drug called LY 255283 can stop certain cells in our blood from making substances that cause inflammation.
Methodology
Human peripheral blood polymorphonuclear leukocytes and monocytes were isolated from healthy volunteers and incubated with LY 255283 before stimulation with calcium ionophore A23187 to measure the production of leukotriene B4 and thromboxane B2.
Limitations
The effects of LY 255283 on 5-lipoxygenase and LTA hydrolase activities were not studied.
Participant Demographics
Healthy volunteers who had abstained from any drugs for at least 1 week.
Statistical Information
P-Value
p<0.0007
Statistical Significance
p<0.05
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