The Role of TCF-1 in Regulating IL-17 Gene Expression
Author Information
Author(s): Ma Jian Wang, Ruiqing Fang, Xianfeng Ding, Yan Sun, Zuoming
Primary Institution: Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California, United States of America
Hypothesis
TCF-1 is required to repress IL-17 gene expression during T cell development.
Conclusion
TCF-1 is critical for maintaining the IL-17 gene locus in a repressive state during T cell development, preventing excessive Th17 differentiation.
Supporting Evidence
- Deletion of TCF-1 resulted in increased IL-17 gene expression in both thymus and peripheral T cells.
- TCF-1-/- mice were more susceptible to Th17-dependent EAE induction.
- TCF-1 does not affect IL-17 production in mature T cells.
Takeaway
TCF-1 helps keep a gene that causes inflammation turned off in developing T cells, so they don't become too active and cause problems.
Methodology
The study involved genetic manipulation of mice to observe the effects of TCF-1 deletion on IL-17 expression and Th17 differentiation.
Limitations
The study was conducted only in mice, and the findings may not directly apply to human T cells.
Participant Demographics
Mice used in the study included TCF-1-/- and wild-type strains.
Digital Object Identifier (DOI)
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