Impact of Cellular miRNAs on Circulating miRNA Biomarker Signatures
Author Information
Author(s): Duttagupta Radha, Jiang Rong, Gollub Jeremy, Getts Robert C., Jones Keith W.
Primary Institution: Applied Research, Affymetrix Inc, Santa Clara, California, United States of America
Hypothesis
The study investigates the pre-analytic considerations for isolating plasma fractions for the study of miRNA biomarkers.
Conclusion
The study highlights the importance of removing cellular miRNA signatures to improve the specificity and reliability of circulating miRNA biomarkers.
Supporting Evidence
- Circulating miRNAs can reflect physiological or disease conditions.
- The study found that different fractionation procedures affect the removal of cellular miRNAs.
- A total of 313 miRNAs were identified as contaminants from cellular sources.
- The S1 fraction showed improved specificity for circulating miRNAs compared to the Cloudy Supernatant.
Takeaway
This study looks at how to better measure tiny molecules called miRNAs in blood, which can help doctors find diseases like cancer more easily.
Methodology
The study involved fractionating whole blood into plasma and cellular components and analyzing miRNA levels using various centrifugation techniques.
Potential Biases
Potential bias due to the specific selection of healthy donors and the exclusion of other demographic groups.
Limitations
The study is limited to a specific demographic (Caucasian males and females) and may not generalize to other populations.
Participant Demographics
17 healthy male Caucasian donors.
Statistical Information
P-Value
<2.3e−12
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website