Molecular Determinants of S100B Oligomer Formation
2011
How S100B Protein Forms Oligomers
publication
Evidence: high
Author Information
Author(s): Thulin Eva, Kesvatera Tõnu, Linse Sara
Primary Institution: Lund University
Hypothesis
S100B oligomerization requires a low molecular weight trigger.
Conclusion
S100B oligomerization requires protonated imidazol as a trigger/cofactor.
Supporting Evidence
- Oligomerization is dependent on both the imidazol concentration and pH.
- Tetramers and hexamers bind the target peptide from p53 with high affinity.
- Imidazol was identified as the substance that promotes formation of tetramers.
Takeaway
S100B is a protein that can stick together to form larger groups, and it needs a special helper called imidazol to do this.
Methodology
The study used highly purified S100B dimers and tested the effects of various conditions and substances on their oligomerization.
Digital Object Identifier (DOI)
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