C5aR1-positive adipocytes mediate non-shivering thermogenesis in neonatal mice
2024

C5aR1-positive adipocytes help newborn mice stay warm

Sample size: 6 publication 10 minutes Evidence: high

Author Information

Author(s): Wang Huan-Yu, Peng Xue-Min, Yang Min, Weng Ying, Yang Xi, Zhan Di, Ning Qin, Luo Xiao-Ping, Chen Yong

Primary Institution: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Hypothesis

C5aR1-positive brown adipocytes are essential for thermoregulation in neonatal mice.

Conclusion

C5aR1-positive brown adipocytes are crucial for maintaining body temperature in newborn mice by regulating thermogenesis.

Supporting Evidence

  • C5aR1 deficiency in brown adipose tissue led to cold intolerance in neonatal mice.
  • Loss of C5aR1 in CD45+ adipocytes increased PF4 secretion, inhibiting the maturity of neighboring brown adipocytes.
  • CD45+ adipocytes exhibited lower thermogenic gene expression compared to classical brown adipocytes.

Takeaway

Some special cells in baby mice help them stay warm by producing heat, and if these cells don't work right, the baby mice get cold.

Methodology

Single-cell RNA sequencing was used to analyze stromal vascular fraction cells from brown adipose tissue of neonatal mice.

Limitations

The in vivo roles of CD45+ adipocytes need further exploration through loss or gain experiments.

Participant Demographics

Neonatal mice (postnatal day 1).

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1016/j.isci.2024.111261

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication