Effects of Tcfap2c on Liver and Intestinal Development in Mice
Author Information
Author(s): Holl Daniel, Kuckenberg Peter, Woynecki Tatiana, Egert Angela, Becker Astrid, Huss Sebastian, Stabenow Dirk, Zimmer Andreas, Knolle Percy, Tolba René, Fischer Hans-Peter, Schorle Hubert
Primary Institution: University of Bonn Medical School
Hypothesis
What are the physiological consequences of Tcfap2c expression in liver and intestine?
Conclusion
Ectopic expression of Tcfap2c leads to liver failure and intestinal dysplasia in mice.
Supporting Evidence
- Transgenic mice with Tcfap2c expression showed rapid mortality within 3-7 days.
- Induction of Tcfap2c led to increased liver enzymes indicative of hepatic failure.
- Histological analysis revealed microvesicular steatosis in hepatocytes.
- Tcfap2c expression resulted in expansion of Sox9 positive progenitor cells in the intestine.
- Gene expression analysis identified 447 common genes deregulated in liver and hepatocyte cultures.
Takeaway
When scientists turned on a gene called Tcfap2c in mice, it made their livers sick and caused problems in their intestines.
Methodology
The study used a gain of function model with doxycycline-inducible Tcfap2c transgenic mice to analyze the effects of Tcfap2c expression.
Limitations
The rapid lethality of the mice limited the ability to observe long-term effects of Tcfap2c expression.
Participant Demographics
Adult C57BL/6J x 129/Sv mice aged 8-16 weeks were used.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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