Immune Cell Response in 2D vs. 3D Cultures of Human Primary Cancer-Associated Fibroblasts
Author Information
Author(s): Yang Jian-Ping, Kulkarni Nikhil Nitin, Yamaji Masashi, Shiraishi Tsubasa, Pham Thang, Do Han, Aiello Nicole, Shaw Michael, Nakamura Toshihiro, Abiru Akiko, Gavva Narender R., Horman Shane R.
Primary Institution: Takeda Development Center Americas, Inc.
Hypothesis
The study investigates how different culturing conditions (2D vs. 3D) affect the immune response of cancer-associated fibroblasts (CAFs).
Conclusion
CAFs cultured in 3D exhibit a more immunosuppressive profile and enhance tumor growth compared to those in 2D cultures.
Supporting Evidence
- CAFs in 3D cultures showed increased expression of immunosuppressive cytokines.
- 3D cultured CAFs enhanced tumor cell growth compared to 2D cultures.
- Monocytes exposed to 3D CAF-conditioned media differentiated into M2-like macrophages.
Takeaway
This study shows that growing cancer cells in 3D is better for understanding how they interact with immune cells than growing them in flat dishes.
Methodology
The study used single-nucleus RNA sequencing and proteomics to analyze CAFs cultured in 2D and 3D environments.
Limitations
The study primarily focuses on in vitro models and may not fully capture the complexity of in vivo tumor environments.
Digital Object Identifier (DOI)
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