Maintenance of Long-Range DNA Interactions after Inhibition of Ongoing RNA Polymerase II Transcription
2008
How Transcription Affects DNA Structure in Cells
publication
10 minutes
Evidence: high
Author Information
Author(s): Robert-Jan Palstra, Marieke Simonis, Petra Klous, Emilie Brasset, Bart Eijkelkamp, Wouter de Laat
Primary Institution: Erasmus MC, Rotterdam, The Netherlands
Hypothesis
Inhibition of transcription changes DNA folding and eliminates looping.
Conclusion
The study shows that long-range DNA interactions are maintained even when transcription is inhibited.
Supporting Evidence
- Transcription inhibition did not affect the interactions between regulatory elements and genes.
- Most long-range interactions were maintained even when transcription was blocked.
- Key transcription factors remained bound to regulatory sites despite the lack of transcription.
Takeaway
Even when cells stop making RNA, the way their DNA is organized doesn't change much.
Methodology
The study used 3C and 4C technology to analyze DNA interactions in cells treated with transcription inhibitors.
Participant Demographics
E14.5 fetal liver cells from mice.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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