Enhanced Immunogenicity of a Pseudotype Baculovirus Vaccine for Foot-and-Mouth Disease in Mice
Author Information
Author(s): Cao Yimei, Lu Zengjun, Sun Pu, Fu Yuanfang, Tian Feipeng, Hao Xiaofang, Bao Huifang, Liu Xiangtao, Liu Zaixin
Primary Institution: Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science
Hypothesis
Can a pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-cell immunogen enhance immunogenicity in mice?
Conclusion
The pseudotype baculovirus with T-cell epitopes showed better immunogenicity than the one without, indicating its potential as a vaccine strategy against FMDV.
Supporting Evidence
- Both recombinant baculoviruses were efficiently transduced and expressed target proteins in BHK-21 cells.
- Mice immunized with the recombinant baculovirus produced FMDV-specific neutralizing antibodies.
- The recombinant baculovirus with T-cell epitopes induced significantly higher IFN-γ production.
Takeaway
Researchers created a new type of vaccine using a virus that helps the body fight foot-and-mouth disease, and it worked better when it included special parts that help T-cells.
Methodology
Two recombinant pseudotype baculoviruses were constructed and their immunogenicity was tested in a mouse model.
Limitations
The inactivated vaccine produced higher neutralizing antibody titers than the recombinant vaccines.
Participant Demographics
Female BALB/c mice, aged six to eight weeks.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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