Neuroblastoma Cells Contain Tumor Initiating Cells Targeted by Oncolytic Virus
Author Information
Author(s): Mahller Yonatan Y., Williams Jon P., Baird William H., Mitton Bryan, Grossheim Jonathan, Saeki Yoshinaga, Cancelas Jose A., Ratner Nancy, Cripe Timothy P.
Primary Institution: Cincinnati Children's Hospital Medical Center
Hypothesis
Neuroblastoma contains tumor initiating cells that can be effectively targeted by an oncolytic virus.
Conclusion
The study suggests that human neuroblastoma contains tumor initiating cells that may be effectively targeted by an oncolytic virus.
Supporting Evidence
- Four of eight human neuroblastoma cell lines formed tumorspheres in neural stem cell media.
- All cell lines contained cells expressing neurogenic stem cell markers including CD133, ABCG2, and nestin.
- Oncolytic viruses may represent an effective therapy for chemotherapy-resistant tumor initiating cells.
Takeaway
Neuroblastoma has special cells that help it grow back after treatment, and a virus designed to attack these cells can stop them from forming tumors.
Methodology
The study involved culturing neuroblastoma cell lines in neural stem cell media, assessing their ability to form tumorspheres, and testing the efficacy of a nestin-targeted oncolytic herpes simplex virus.
Limitations
The study used cell lines derived from neuroblastomas, which may not fully represent primary human samples.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website