Tumour necrosis factor-a induces an increase in susceptibility of human glioblastoma U87-MG cells to natural killer cell-mediated lysis
1994
TNF-a Increases Susceptibility of Glioblastoma Cells to NK Cell Lysis
publication
Evidence: moderate
Author Information
Author(s): S. Kondo, D. Yin, J. Takeuchi, T. Morimura, S.I. Miyatake, S. Nakatsu, Y. Oda, H. Kikuchi
Primary Institution: Utano National Hospital; Kyoto University
Hypothesis
Does TNF-a treatment increase the susceptibility of glioblastoma cells to lysis by natural killer cells?
Conclusion
TNF-a treatment enhances the susceptibility of glioblastoma cells to lysis by natural killer cells primarily through increased expression of ICAM-1.
Supporting Evidence
- TNF-a treatment for 48 hours significantly increased the susceptibility of U87-MG cells to NK cell lysis.
- Increased expression of ICAM-1 was observed in TNF-a treated U87-MG cells.
- Antisense ICAM-1 oligonucleotide inhibited NK cell-mediated lysis of TNF-a treated cells.
- Acid treatment following TNF-a treatment increased lysis by NK cells.
Takeaway
When glioblastoma cells are treated with a substance called TNF-a, they become easier for certain immune cells to destroy.
Methodology
U87-MG glioblastoma cells were treated with TNF-a and their susceptibility to lysis by NK cells was measured using various assays.
Statistical Information
P-Value
p<0.1
Statistical Significance
p<0.05
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