Effects of anti-inflammatory drugs on fever and neutrophilia induced by Clostridium difficile toxin B
Author Information
Author(s): R. A. Cardoso, A. A. Melo Filho, M. C. C. Melo, D. M. Lyerly, T. D. Wilkins, A. A. M. Lima, R. A. Ribeiro, G. E. P. Souza
Primary Institution: Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, USP, Brazil
Hypothesis
This study aimed to investigate the pyrogenic activity and the ability to induce neutrophilia of the C. difficile toxin B in rats.
Conclusion
Clostridium difficile toxin B induces fever and neutrophilia in rats, with the febrile response being inhibited by dexamethasone and indomethacin.
Supporting Evidence
- Intravenous injection of toxin B induced a dose-dependent increase in body temperature.
- The febrile response to the highest dose of toxin B peaked at 5 hours and returned to baseline after 24 hours.
- Dexamethasone significantly reduced the febrile response induced by toxin B.
- Indomethacin did not affect the neutrophilia induced by toxin B.
Takeaway
The study found that a toxin from a bacteria can make rats have a fever and more white blood cells, and some medicines can help reduce the fever.
Methodology
Wistar rats were injected with varying doses of Clostridium difficile toxin B, and their body temperature and neutrophil counts were measured.
Limitations
The study did not explore the long-term effects of the toxin or the potential for other confounding factors.
Participant Demographics
Wistar rats weighing 180-200g were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website