Regulation of Mouse Alpha A-Crystallin Gene Expression
Author Information
Author(s): Wolf Louise, Yang Ying, Wawrousek Eric, Cvekl Ales
Primary Institution: Albert Einstein College of Medicine
Hypothesis
Additional cis regulatory elements are required to recapitulate endogenous αA-crystallin expression.
Conclusion
A 148 kb αA-BAC contains all regulatory regions required for αA-crystallin expression in the lens, but not in other tissues.
Supporting Evidence
- EGFP expression was first detected in the lens pit at E10.5.
- Transgene expression patterns were similar to those of endogenous αA-crystallin.
- Deletion of DCR3 did not affect early αA-crystallin expression.
Takeaway
The study shows how a specific gene is turned on in the eye's lens during development, and that certain parts of the gene are important for this process.
Methodology
Bacterial artificial chromosome (BAC) and standard transgenic approaches were used to examine the regulation of the mouse Cryaa gene.
Limitations
The study did not explore the effects of DCR3 deletion on expression in all transgenic lines.
Digital Object Identifier (DOI)
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