The Putative bZIP Transcription Factor BzpN Slows Proliferation and Functions in the Regulation of Cell Density by Autocrine Signals in Dictyostelium
2011
BzpN Transcription Factor Slows Cell Growth in Dictyostelium
publication
Evidence: moderate
Author Information
Author(s): Jonathan E. Phillips, Eryong Huang, Gad Shaulsky, Richard H. Gomer
Primary Institution: Rice University, Baylor College of Medicine, Texas A&M University
Hypothesis
BzpN plays a role in the inhibition of proliferation by the autocrine signals AprA and CfaD in Dictyostelium discoideum.
Conclusion
BzpN is necessary for the inhibition of cell proliferation by the autocrine signals AprA and CfaD.
Supporting Evidence
- BzpN null mutants proliferate more rapidly than wild-type cells.
- Recombinant AprA inhibits wild-type cell proliferation but not that of bzpN− cells.
- Overexpression of BzpN reduces cell density at stationary phase.
Takeaway
BzpN helps control how fast cells grow in a type of slime mold, and without it, the cells grow too quickly.
Methodology
The study involved creating a BzpN null mutant and analyzing its proliferation and response to recombinant AprA and CfaD.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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