Changes in ATP Release and Metabolism in Nerve Injury
Author Information
Author(s): Matsuka Yoshizo, Ono Takeshi, Iwase Hirotate, Mitrirattanakul Somsak, Omoto Kevin S, Cho Ting, Lam Yan Yan N, Snyder Bradley, Spigelman Igor
Primary Institution: University of California, Los Angeles
Hypothesis
The differential effects of sciatic nerve entrapment on basal and evoked ATP release could result from the conversion of extracellular ATP to adenosine with subsequent activation of adenosine A1 receptors on DRG neurons.
Conclusion
Peripheral nerve entrapment increases DRG metabolism and ATP release, which is modulated by increased A1R activation.
Supporting Evidence
- Rats with nerve injury showed increased sensitivity to mechanical and thermal stimuli.
- Basal ATP levels were significantly higher in injured DRG compared to control.
- KCl-evoked ATP release was absent in DRG ipsilateral to nerve injury.
- Selective A1R blockade increased basal ATP levels and restored KCl-evoked ATP release.
Takeaway
When a nerve is injured, the cells around it release more ATP, which is important for pain signaling, but the way they release it changes.
Methodology
The study involved measuring ATP release from dorsal root ganglia in rats after inducing neuropathy through sciatic nerve entrapment.
Limitations
The study does not differentiate between contributions of various cell types to ATP release and metabolism.
Participant Demographics
Adult male Sprague-Dawley rats weighing 200–250 g were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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