Phthiocerol Dimycocerosates of M. tuberculosis Participate in Macrophage Invasion by Inducing Changes in the Organization of Plasma Membrane Lipids
2009

How a Bacteria Uses Lipids to Invade Immune Cells

publication Evidence: high

Author Information

Author(s): Catherine Astarie-Dequeker, Laurent Le Guyader, Wladimir Malaga, Fam-Ky Seaphanh, Christian Chalut, André Lopez, Christophe Guilhot

Primary Institution: CNRS, Institut de Pharmacologie et de Biologie Structurale (IPBS), Toulouse, France

Hypothesis

Do phthiocerol dimycocerosates (DIM) from Mycobacterium tuberculosis play a role in macrophage invasion by altering plasma membrane lipid organization?

Conclusion

DIM are crucial for the efficient phagocytosis of Mycobacterium tuberculosis by macrophages and help prevent the acidification of phagosomes.

Supporting Evidence

  • DIM are major virulence factors of Mycobacterium tuberculosis.
  • DIM deficiency impaired the capacity of Mtb to infect macrophages.
  • Coating DIM-less mutants with DIM restored their ability to infect macrophages.

Takeaway

Some bacteria have special fats that help them sneak into our body's defense cells, making it harder for our body to fight them off.

Methodology

The study used M. tuberculosis mutants and macrophage infection assays to analyze the role of DIM in phagocytosis and phagosome maturation.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.ppat.1000289

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