Ouabain and SFK Signaling in Mouse Blastocyst Development
Author Information
Author(s): Giannatselis Holly, Calder Michele, Watson Andrew J.
Primary Institution: The University of Western Ontario
Hypothesis
Ouabain-mediated, SRC-activated pathways play an important role in regulating preimplantation development by regulating trophectoderm tight junction function.
Conclusion
SFK activity is necessary for blastocyst formation and regulates trophectoderm tight junction permeability.
Supporting Evidence
- SFK activity is required for blastocyst formation.
- Ouabain treatment affects SRC phosphorylation at the blastocyst stage.
- High ouabain concentrations reduce SRC activation, while low concentrations increase it.
- SFK inhibition increases trophectoderm tight junction permeability.
Takeaway
This study shows that a substance called ouabain helps mouse embryos develop properly by affecting how cells stick together.
Methodology
Embryos were treated with ouabain and SFK inhibitors, and their development and tight junction permeability were assessed.
Limitations
The study did not measure cell numbers in recovered blastocysts after treatment.
Participant Demographics
CD1 and MF1 female mice were used for embryo collection.
Statistical Information
P-Value
p≤0.05
Statistical Significance
p≤0.05
Digital Object Identifier (DOI)
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