Dietary Cholesterol Reduces Plasma Triacylglycerol in Apolipoprotein E-Null Mice: Suppression of Lipin-1 and -2 in the Glycerol-3-Phosphate Pathway Cholesterol-Dependent Regulation of TG Synthesis
2011

Cholesterol Reduces Plasma Triglycerides in Mice

Sample size: 3 publication 10 minutes Evidence: moderate

Author Information

Author(s): Obama Takashi, Nagaoka Sayaka, Akagi Kazuki, Kato Rina, Horiuchi Naomi, Horai Yasushi, Aiuchi Toshihiro, Arata Satoru, Yamaguchi Tomohiro, Watanabe Mitsuhiro, Itabe Hiroyuki

Primary Institution: Department of Biological Chemistry, Showa University School of Pharmacy, Shinagawa, Tokyo, Japan

Hypothesis

How does dietary cholesterol affect triglyceride metabolism in the liver of apolipoprotein E-null mice?

Conclusion

Cholesterol and its metabolites regulate triglyceride metabolism by suppressing lipin-1 and lipin-2 in the liver.

Supporting Evidence

  • Cholesterol intake increased bile acids in the liver.
  • Triglyceride content in VLDL was reduced by 78% in high-cholesterol diet mice.
  • Lipin-1 and lipin-2 expression was significantly suppressed in the liver.

Takeaway

When mice that can't process cholesterol well eat a lot of cholesterol, their bodies make less fat in the blood.

Methodology

ApoE-KO mice were fed a high-cholesterol diet, and various analyses were performed to assess lipid metabolism and gene expression.

Limitations

The study was conducted on a specific mouse model, which may not fully represent human metabolism.

Participant Demographics

ApoE-KO mice, a model for studying cholesterol metabolism.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0022917

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